Construct: shRNA TRCN0000095992

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172622.1-3165s1c1
DNA Barcode:
AGGCAACTTCATTTGTGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trerf1 (224829)
Gene Description:
transcriptional regulating factor 1
Transcript:
RefSeq NM_172622.1 (NON-CURRENT)
Match location:
Position 3165 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000095992)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGCAACTTCATTTGTGAAAT
Hairpin Sequence:
5'-CCGG-AGGCAACTTCATTTGTGAAAT-CTCGAG-ATTTCACAAATGAAGTTGCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGCAACTTCATTTGTGAAATCTCGAGATTTCACAAATGAAGTTGCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGCAACTTCATTTGTGAAATCTCGAGATTTCACAAATGAAGTTGCCT-3'

Other clones with same target sequence:

(none)