Construct: shRNA TRCN0000096028

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001004167.1-531s1c1
DNA Barcode:
TCACTGTTATAGGCTACACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm5433 (432637)
Gene Description:
predicted gene 5433
Transcript:
RefSeq NM_001004167.1 (NON-CURRENT)
Match location:
Position 531 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000096028)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCACTGTTATAGGCTACACTT
Hairpin Sequence:
5'-CCGG-TCACTGTTATAGGCTACACTT-CTCGAG-AAGTGTAGCCTATAACAGTGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCACTGTTATAGGCTACACTTCTCGAGAAGTGTAGCCTATAACAGTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCACTGTTATAGGCTACACTTCTCGAGAAGTGTAGCCTATAACAGTGA-3'

Other clones with same target sequence:

(none)