Construct: shRNA TRCN0000096049

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008543.1-2820s1c1
DNA Barcode:
GCAAACTCTTTGGTTGGTGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smad7 (17131)
Gene Description:
SMAD family member 7
Transcript:
RefSeq NM_008543.1 (NON-CURRENT)
Match location:
Position 2820 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000096049)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAACTCTTTGGTTGGTGTT
Hairpin Sequence:
5'-CCGG-GCAAACTCTTTGGTTGGTGTT-CTCGAG-AACACCAACCAAAGAGTTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAACTCTTTGGTTGGTGTTCTCGAGAACACCAACCAAAGAGTTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAACTCTTTGGTTGGTGTTCTCGAGAACACCAACCAAAGAGTTTGC-3'

Other clones with same target sequence:

(none)