Construct: shRNA TRCN0000096079

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177596.2-2212s1c1
DNA Barcode:
GCAGCAATGAAGGAAATCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp947 (210853)
Gene Description:
zinc finger protein 947
Transcript:
RefSeq NM_177596.2 (NON-CURRENT)
Match location:
Position 2212 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000096079)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCAATGAAGGAAATCTTT
Hairpin Sequence:
5'-CCGG-GCAGCAATGAAGGAAATCTTT-CTCGAG-AAAGATTTCCTTCATTGCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCAATGAAGGAAATCTTTCTCGAGAAAGATTTCCTTCATTGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCAATGAAGGAAATCTTTCTCGAGAAAGATTTCCTTCATTGCTGC-3'

Other clones with same target sequence:

(none)