Construct: shRNA TRCN0000096144

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008130.1-4485s1c1
DNA Barcode:
GCAGAATTATTCCGGTCAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gli3 (14634)
Gene Description:
GLI-Kruppel family member GLI3
Transcript:
RefSeq NM_008130.1 (NON-CURRENT)
Match location:
Position 4485 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000096144)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAATTATTCCGGTCAGTT
Hairpin Sequence:
5'-CCGG-GCAGAATTATTCCGGTCAGTT-CTCGAG-AACTGACCGGAATAATTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAATTATTCCGGTCAGTTCTCGAGAACTGACCGGAATAATTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAATTATTCCGGTCAGTTCTCGAGAACTGACCGGAATAATTCTGC-3'

Other clones with same target sequence:

(none)