Construct: shRNA TRCN0000096237

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021399.1-436s1c1
DNA Barcode:
CCTGATGAAGATGACCACCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bcl11b (58208)
Gene Description:
B cell leukemia/lymphoma 11B
Transcript:
RefSeq NM_021399.1 (NON-CURRENT)
Match location:
Position 436 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000096237)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGATGAAGATGACCACCTA
Hairpin Sequence:
5'-CCGG-CCTGATGAAGATGACCACCTA-CTCGAG-TAGGTGGTCATCTTCATCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGATGAAGATGACCACCTACTCGAGTAGGTGGTCATCTTCATCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGATGAAGATGACCACCTACTCGAGTAGGTGGTCATCTTCATCAGG-3'

Other clones with same target sequence:

(none)