Construct: shRNA TRCN0000096422

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_358939.1-138s1c1
DNA Barcode:
CGCCGAGCTAGACGAACCCGA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gsc2 (195333)
Gene Description:
goosecoid homebox 2
Transcript:
RefSeq XM_358939.1 (NON-CURRENT)
Match location:
Position 138 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000096422)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCGAGCTAGACGAACCCGA
Hairpin Sequence:
5'-CCGG-CGCCGAGCTAGACGAACCCGA-CTCGAG-TCGGGTTCGTCTAGCTCGGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCGAGCTAGACGAACCCGACTCGAGTCGGGTTCGTCTAGCTCGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCGAGCTAGACGAACCCGACTCGAGTCGGGTTCGTCTAGCTCGGCG-3'

Other clones with same target sequence:

(none)