Construct: shRNA TRCN0000096429

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009824.1-2446s1c1
DNA Barcode:
GCAAACAAGCTACTTAGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cbfa2t3 (12398)
Gene Description:
core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human)
Transcript:
RefSeq NM_009824.1 (NON-CURRENT)
Match location:
Position 2446 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000096429)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAACAAGCTACTTAGAAAT
Hairpin Sequence:
5'-CCGG-GCAAACAAGCTACTTAGAAAT-CTCGAG-ATTTCTAAGTAGCTTGTTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAACAAGCTACTTAGAAATCTCGAGATTTCTAAGTAGCTTGTTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAACAAGCTACTTAGAAATCTCGAGATTTCTAAGTAGCTTGTTTGC-3'

Other clones with same target sequence:

(none)