Construct: shRNA TRCN0000096462

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011772.1-1518s1c1
DNA Barcode:
CGGCCAACTTTCATTGATCGT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ikzf4 (22781)
Gene Description:
IKAROS family zinc finger 4
Transcript:
RefSeq NM_011772.1 (NON-CURRENT)
Match location:
Position 1518 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000096462)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCCAACTTTCATTGATCGT
Hairpin Sequence:
5'-CCGG-CGGCCAACTTTCATTGATCGT-CTCGAG-ACGATCAATGAAAGTTGGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCCAACTTTCATTGATCGTCTCGAGACGATCAATGAAAGTTGGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCCAACTTTCATTGATCGTCTCGAGACGATCAATGAAAGTTGGCCG-3'

Other clones with same target sequence:

(none)