Construct: shRNA TRCN0000096467

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021521.1-3583s1c1
DNA Barcode:
GCTGTGTTTGTACTCGGAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Med12 (59024)
Gene Description:
mediator complex subunit 12
Transcript:
RefSeq NM_021521.1 (NON-CURRENT)
Match location:
Position 3583 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000096467)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTGTTTGTACTCGGAGAT
Hairpin Sequence:
5'-CCGG-GCTGTGTTTGTACTCGGAGAT-CTCGAG-ATCTCCGAGTACAAACACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTGTTTGTACTCGGAGATCTCGAGATCTCCGAGTACAAACACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTGTTTGTACTCGGAGATCTCGAGATCTCCGAGTACAAACACAGC-3'

Other clones with same target sequence:

(none)