Construct: shRNA TRCN0000096513

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011766.2-2363s1c1
DNA Barcode:
CCTTCAAACATCCTGCATCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfpm2 (22762)
Gene Description:
zinc finger protein, multitype 2
Transcript:
RefSeq NM_011766.2 (NON-CURRENT)
Match location:
Position 2363 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000096513)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCAAACATCCTGCATCAA
Hairpin Sequence:
5'-CCGG-CCTTCAAACATCCTGCATCAA-CTCGAG-TTGATGCAGGATGTTTGAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCAAACATCCTGCATCAACTCGAGTTGATGCAGGATGTTTGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCAAACATCCTGCATCAACTCGAGTTGATGCAGGATGTTTGAAGG-3'

Other clones with same target sequence:

(none)