Construct: shRNA TRCN0000096565

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144910.1-684s1c1
DNA Barcode:
CCTGGGCATTAAACTGGGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cnot6l (231464)
Gene Description:
CCR4-NOT transcription complex, subunit 6-like
Transcript:
RefSeq NM_144910.1 (NON-CURRENT)
Match location:
Position 684 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000096565)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGGCATTAAACTGGGAAT
Hairpin Sequence:
5'-CCGG-CCTGGGCATTAAACTGGGAAT-CTCGAG-ATTCCCAGTTTAATGCCCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGGCATTAAACTGGGAATCTCGAGATTCCCAGTTTAATGCCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGGCATTAAACTGGGAATCTCGAGATTCCCAGTTTAATGCCCAGG-3'

Other clones with same target sequence:

(none)