Construct: shRNA TRCN0000096589

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026949.2-1815s1c1
DNA Barcode:
CCACAGAAAGAAAGGACAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cnot8 (69125)
Gene Description:
CCR4-NOT transcription complex, subunit 8
Transcript:
RefSeq NM_026949.2 (NON-CURRENT)
Match location:
Position 1815 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000096589)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACAGAAAGAAAGGACAATT
Hairpin Sequence:
5'-CCGG-CCACAGAAAGAAAGGACAATT-CTCGAG-AATTGTCCTTTCTTTCTGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACAGAAAGAAAGGACAATTCTCGAGAATTGTCCTTTCTTTCTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACAGAAAGAAAGGACAATTCTCGAGAATTGTCCTTTCTTTCTGTGG-3'

Other clones with same target sequence:

(none)