Construct: shRNA TRCN0000096643

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021878.2-1430s1c1
DNA Barcode:
GAACCCAAAGTCATGCACTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Jarid2 (16468)
Gene Description:
jumonji, AT rich interactive domain 2
Transcript:
RefSeq NM_021878.2 (NON-CURRENT)
Match location:
Position 1430 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000096643)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACCCAAAGTCATGCACTAA
Hairpin Sequence:
5'-CCGG-GAACCCAAAGTCATGCACTAA-CTCGAG-TTAGTGCATGACTTTGGGTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACCCAAAGTCATGCACTAACTCGAGTTAGTGCATGACTTTGGGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACCCAAAGTCATGCACTAACTCGAGTTAGTGCATGACTTTGGGTTC-3'

Other clones with same target sequence:

(none)