Construct: shRNA TRCN0000096647

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030147.2-2037s1c1
DNA Barcode:
GCTGAGATAGTAGCTGGAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Brd8 (78656)
Gene Description:
bromodomain containing 8
Transcript:
RefSeq NM_030147.2 (NON-CURRENT)
Match location:
Position 2037 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000096647)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAGATAGTAGCTGGAGTT
Hairpin Sequence:
5'-CCGG-GCTGAGATAGTAGCTGGAGTT-CTCGAG-AACTCCAGCTACTATCTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAGATAGTAGCTGGAGTTCTCGAGAACTCCAGCTACTATCTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAGATAGTAGCTGGAGTTCTCGAGAACTCCAGCTACTATCTCAGC-3'

Other clones with same target sequence:

  1. TRCN0000301664