Construct: shRNA TRCN0000096818

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172703.2-2675s1c1
DNA Barcode:
CACTTGAGAAAGGCGGAGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Eif4g3 (230861)
Gene Description:
eukaryotic translation initiation factor 4 gamma, 3
Transcript:
RefSeq NM_172703.2 (NON-CURRENT)
Match location:
Position 2675 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000096818)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACTTGAGAAAGGCGGAGAAT
Hairpin Sequence:
5'-CCGG-CACTTGAGAAAGGCGGAGAAT-CTCGAG-ATTCTCCGCCTTTCTCAAGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACTTGAGAAAGGCGGAGAATCTCGAGATTCTCCGCCTTTCTCAAGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACTTGAGAAAGGCGGAGAATCTCGAGATTCTCCGCCTTTCTCAAGTG-3'

Other clones with same target sequence:

(none)