Construct: shRNA TRCN0000096854

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025840.2-1720s1c1
DNA Barcode:
GCCACTTCAATAGTTGTGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bzw2 (66912)
Gene Description:
basic leucine zipper and W2 domains 2
Transcript:
RefSeq NM_025840.2 (NON-CURRENT)
Match location:
Position 1720 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000096854)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACTTCAATAGTTGTGTTT
Hairpin Sequence:
5'-CCGG-GCCACTTCAATAGTTGTGTTT-CTCGAG-AAACACAACTATTGAAGTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACTTCAATAGTTGTGTTTCTCGAGAAACACAACTATTGAAGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACTTCAATAGTTGTGTTTCTCGAGAAACACAACTATTGAAGTGGC-3'

Other clones with same target sequence:

(none)