Construct: shRNA TRCN0000096864

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011291.2-159s1c1
DNA Barcode:
GCGAAGGAATTTCGCAGAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rpl7 (19989)
Gene Description:
ribosomal protein L7
Transcript:
RefSeq NM_011291.2 (NON-CURRENT)
Match location:
Position 159 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000096864)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGAAGGAATTTCGCAGAGTT
Hairpin Sequence:
5'-CCGG-GCGAAGGAATTTCGCAGAGTT-CTCGAG-AACTCTGCGAAATTCCTTCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGAAGGAATTTCGCAGAGTTCTCGAGAACTCTGCGAAATTCCTTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGAAGGAATTTCGCAGAGTTCTCGAGAACTCTGCGAAATTCCTTCGC-3'

Other clones with same target sequence:

(none)