Construct: shRNA TRCN0000096887

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153547.2-281s1c1
DNA Barcode:
GCTCCCTTTAAAGAGGCTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gnl3 (30877)
Gene Description:
guanine nucleotide binding protein-like 3 (nucleolar)
Transcript:
RefSeq NM_153547.2 (NON-CURRENT)
Match location:
Position 281 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000096887)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCCCTTTAAAGAGGCTCTT
Hairpin Sequence:
5'-CCGG-GCTCCCTTTAAAGAGGCTCTT-CTCGAG-AAGAGCCTCTTTAAAGGGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCCCTTTAAAGAGGCTCTTCTCGAGAAGAGCCTCTTTAAAGGGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCCCTTTAAAGAGGCTCTTCTCGAGAAGAGCCTCTTTAAAGGGAGC-3'

Other clones with same target sequence:

(none)