Construct: shRNA TRCN0000096897

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025824.2-577s1c1
DNA Barcode:
CCTTAATGCATCCATTCTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bzw1 (66882)
Gene Description:
basic leucine zipper and W2 domains 1
Transcript:
RefSeq NM_025824.2 (NON-CURRENT)
Match location:
Position 577 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000096897)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTAATGCATCCATTCTTAA
Hairpin Sequence:
5'-CCGG-CCTTAATGCATCCATTCTTAA-CTCGAG-TTAAGAATGGATGCATTAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTAATGCATCCATTCTTAACTCGAGTTAAGAATGGATGCATTAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTAATGCATCCATTCTTAACTCGAGTTAAGAATGGATGCATTAAGG-3'

Other clones with same target sequence:

  1. TRCN0000312065