Construct: shRNA TRCN0000096928

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207000.1-420s1c1
DNA Barcode:
GCTACTGAAAGGAGTGACTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Macroh2a2 (404634)
Gene Description:
macroH2A.2 histone
Transcript:
RefSeq NM_207000.1 (NON-CURRENT)
Match location:
Position 420 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000096928)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTACTGAAAGGAGTGACTAT
Hairpin Sequence:
5'-CCGG-GCTACTGAAAGGAGTGACTAT-CTCGAG-ATAGTCACTCCTTTCAGTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTACTGAAAGGAGTGACTATCTCGAGATAGTCACTCCTTTCAGTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTACTGAAAGGAGTGACTATCTCGAGATAGTCACTCCTTTCAGTAGC-3'

Other clones with same target sequence:

(none)