Construct: shRNA TRCN0000096935

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015787.2-641s1c1
DNA Barcode:
GTAAAGCCTAAAGCGGCCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hist1h1e (50709)
Gene Description:
histone cluster 1, H1e
Transcript:
RefSeq NM_015787.2 (NON-CURRENT)
Match location:
Position 641 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000096935)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAAAGCCTAAAGCGGCCAAA
Hairpin Sequence:
5'-CCGG-GTAAAGCCTAAAGCGGCCAAA-CTCGAG-TTTGGCCGCTTTAGGCTTTAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAAAGCCTAAAGCGGCCAAACTCGAGTTTGGCCGCTTTAGGCTTTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAAAGCCTAAAGCGGCCAAACTCGAGTTTGGCCGCTTTAGGCTTTAC-3'

Other clones with same target sequence:

(none)