Construct: shRNA TRCN0000096944

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178194.2-698s1c1
DNA Barcode:
CCACCTTTATATGGTTCTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hist1h2be (319179)
Gene Description:
histone cluster 1, H2be
Transcript:
RefSeq NM_178194.2 (NON-CURRENT)
Match location:
Position 698 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000096944)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACCTTTATATGGTTCTTTA
Hairpin Sequence:
5'-CCGG-CCACCTTTATATGGTTCTTTA-CTCGAG-TAAAGAACCATATAAAGGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACCTTTATATGGTTCTTTACTCGAGTAAAGAACCATATAAAGGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACCTTTATATGGTTCTTTACTCGAGTAAAGAACCATATAAAGGTGG-3'

Other clones with same target sequence:

(none)