Construct: shRNA TRCN0000097013

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178218.2-219s1c1
DNA Barcode:
GCTAGAATATCTGACTGCTGA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hist3h2a (319162)
Gene Description:
histone cluster 3, H2a
Transcript:
RefSeq NM_178218.2 (NON-CURRENT)
Match location:
Position 219 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000097013)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAGAATATCTGACTGCTGA
Hairpin Sequence:
5'-CCGG-GCTAGAATATCTGACTGCTGA-CTCGAG-TCAGCAGTCAGATATTCTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAGAATATCTGACTGCTGACTCGAGTCAGCAGTCAGATATTCTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAGAATATCTGACTGCTGACTCGAGTCAGCAGTCAGATATTCTAGC-3'

Other clones with same target sequence:

(none)