Construct: shRNA TRCN0000097037

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177688.2-116s1c1
DNA Barcode:
GCTGCTGCGGAAGGGCAACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
H2afj (232440)
Gene Description:
H2A histone family, member J
Transcript:
RefSeq NM_177688.2 (NON-CURRENT)
Match location:
Position 116 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000097037)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCTGCGGAAGGGCAACTA
Hairpin Sequence:
5'-CCGG-GCTGCTGCGGAAGGGCAACTA-CTCGAG-TAGTTGCCCTTCCGCAGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCTGCGGAAGGGCAACTACTCGAGTAGTTGCCCTTCCGCAGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCTGCGGAAGGGCAACTACTCGAGTAGTTGCCCTTCCGCAGCAGC-3'

Other clones with same target sequence:

(none)