Construct: shRNA TRCN0000097099

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183191.1-5852s1c1
DNA Barcode:
CCGACTTTAATCCCAGTAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plch1 (269437)
Gene Description:
phospholipase C, eta 1
Transcript:
RefSeq NM_183191.1 (NON-CURRENT)
Match location:
Position 5852 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000097099)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGACTTTAATCCCAGTAGAA
Hairpin Sequence:
5'-CCGG-CCGACTTTAATCCCAGTAGAA-CTCGAG-TTCTACTGGGATTAAAGTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGACTTTAATCCCAGTAGAACTCGAGTTCTACTGGGATTAAAGTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGACTTTAATCCCAGTAGAACTCGAGTTCTACTGGGATTAAAGTCGG-3'

Other clones with same target sequence:

(none)