Construct: shRNA TRCN0000097129

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010901.1-3407s1c1
DNA Barcode:
GTGGGAAACGAGCTTTGCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nfatc3 (18021)
Gene Description:
nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 3
Transcript:
RefSeq NM_010901.1 (NON-CURRENT)
Match location:
Position 3407 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000097129)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGGGAAACGAGCTTTGCTTT
Hairpin Sequence:
5'-CCGG-GTGGGAAACGAGCTTTGCTTT-CTCGAG-AAAGCAAAGCTCGTTTCCCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGGGAAACGAGCTTTGCTTTCTCGAGAAAGCAAAGCTCGTTTCCCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGGGAAACGAGCTTTGCTTTCTCGAGAAAGCAAAGCTCGTTTCCCAC-3'

Other clones with same target sequence:

(none)