Construct: shRNA TRCN0000097139

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153514.3-3591s1c1
DNA Barcode:
CCTCTATTATTATGTGGCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rhobtb2 (246710)
Gene Description:
Rho-related BTB domain containing 2
Transcript:
RefSeq NM_153514.3 (NON-CURRENT)
Match location:
Position 3591 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000097139)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTATTATTATGTGGCTAT
Hairpin Sequence:
5'-CCGG-CCTCTATTATTATGTGGCTAT-CTCGAG-ATAGCCACATAATAATAGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTATTATTATGTGGCTATCTCGAGATAGCCACATAATAATAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTATTATTATGTGGCTATCTCGAGATAGCCACATAATAATAGAGG-3'

Other clones with same target sequence:

(none)