Construct: shRNA TRCN0000097151

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177568.2-1321s1c1
DNA Barcode:
CCTGTCATCCTATCATTTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plcb2 (18796)
Gene Description:
phospholipase C, beta 2
Transcript:
RefSeq NM_177568.2 (CURRENT)
Match location:
Position 1321 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000097151)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTCATCCTATCATTTGAA
Hairpin Sequence:
5'-CCGG-CCTGTCATCCTATCATTTGAA-CTCGAG-TTCAAATGATAGGATGACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTCATCCTATCATTTGAACTCGAGTTCAAATGATAGGATGACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTCATCCTATCATTTGAACTCGAGTTCAAATGATAGGATGACAGG-3'

Other clones with same target sequence:

(none)