Construct: shRNA TRCN0000097171

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_054066.2-909s1c1
DNA Barcode:
GCAATAAACAAGTATGCCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plcz1 (114875)
Gene Description:
phospholipase C, zeta 1
Transcript:
RefSeq NM_054066.2 (NON-CURRENT)
Match location:
Position 909 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000097171)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAATAAACAAGTATGCCTTT
Hairpin Sequence:
5'-CCGG-GCAATAAACAAGTATGCCTTT-CTCGAG-AAAGGCATACTTGTTTATTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAATAAACAAGTATGCCTTTCTCGAGAAAGGCATACTTGTTTATTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAATAAACAAGTATGCCTTTCTCGAGAAAGGCATACTTGTTTATTGC-3'

Other clones with same target sequence:

(none)