Construct: shRNA TRCN0000097186

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198420.1-3161s1c1
DNA Barcode:
CCTCATGAATTCTACGAACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arhgap39 (223666)
Gene Description:
Rho GTPase activating protein 39
Transcript:
RefSeq NM_198420.1 (NON-CURRENT)
Match location:
Position 3161 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000097186)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCATGAATTCTACGAACAA
Hairpin Sequence:
5'-CCGG-CCTCATGAATTCTACGAACAA-CTCGAG-TTGTTCGTAGAATTCATGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCATGAATTCTACGAACAACTCGAGTTGTTCGTAGAATTCATGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCATGAATTCTACGAACAACTCGAGTTGTTCGTAGAATTCATGAGG-3'

Other clones with same target sequence:

(none)