Construct: shRNA TRCN0000097251

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175256.4-2177s1c1
DNA Barcode:
CGAGCATGTGAAGATGGATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Heg1 (77446)
Gene Description:
heart development protein with EGF-like domains 1
Transcript:
RefSeq NM_175256.4 (NON-CURRENT)
Match location:
Position 2177 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000097251)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGCATGTGAAGATGGATAT
Hairpin Sequence:
5'-CCGG-CGAGCATGTGAAGATGGATAT-CTCGAG-ATATCCATCTTCACATGCTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGCATGTGAAGATGGATATCTCGAGATATCCATCTTCACATGCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGCATGTGAAGATGGATATCTCGAGATATCCATCTTCACATGCTCG-3'

Other clones with same target sequence:

(none)