Construct: shRNA TRCN0000097258

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_284495.2-308s1c1
DNA Barcode:
GCCTATCTCATCGAGATTGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dixdc1 (330938)
Gene Description:
DIX domain containing 1
Transcript:
RefSeq XM_284495.2 (NON-CURRENT)
Match location:
Position 308 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000097258)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTATCTCATCGAGATTGTT
Hairpin Sequence:
5'-CCGG-GCCTATCTCATCGAGATTGTT-CTCGAG-AACAATCTCGATGAGATAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTATCTCATCGAGATTGTTCTCGAGAACAATCTCGATGAGATAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTATCTCATCGAGATTGTTCTCGAGAACAATCTCGATGAGATAGGC-3'

Other clones with same target sequence:

(none)