Construct: shRNA TRCN0000097328

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001025571.1-189s1c1
DNA Barcode:
GCCCAGCATGCAAGTTATGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sh2d2a (27371)
Gene Description:
SH2 domain containing 2A
Transcript:
RefSeq NM_001025571.1 (NON-CURRENT)
Match location:
Position 189 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000097328)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCAGCATGCAAGTTATGAA
Hairpin Sequence:
5'-CCGG-GCCCAGCATGCAAGTTATGAA-CTCGAG-TTCATAACTTGCATGCTGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCAGCATGCAAGTTATGAACTCGAGTTCATAACTTGCATGCTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCAGCATGCAAGTTATGAACTCGAGTTCATAACTTGCATGCTGGGC-3'

Other clones with same target sequence:

(none)