Construct: shRNA TRCN0000097335

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146061.3-1258s1c1
DNA Barcode:
GAAGGGATATTCATTGACTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prr5 (109270)
Gene Description:
proline rich 5 (renal)
Transcript:
RefSeq NM_146061.3 (NON-CURRENT)
Match location:
Position 1258 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000097335)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGGGATATTCATTGACTTT
Hairpin Sequence:
5'-CCGG-GAAGGGATATTCATTGACTTT-CTCGAG-AAAGTCAATGAATATCCCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGGGATATTCATTGACTTTCTCGAGAAAGTCAATGAATATCCCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGGGATATTCATTGACTTTCTCGAGAAAGTCAATGAATATCCCTTC-3'

Other clones with same target sequence:

(none)