Construct: shRNA TRCN0000097369

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028142.1-1483s1c1
DNA Barcode:
GCCCATAGTTTAAGCTGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nsun4 (72181)
Gene Description:
NOL1/NOP2/Sun domain family, member 4
Transcript:
RefSeq NM_028142.1 (NON-CURRENT)
Match location:
Position 1483 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000097369)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCATAGTTTAAGCTGTAAA
Hairpin Sequence:
5'-CCGG-GCCCATAGTTTAAGCTGTAAA-CTCGAG-TTTACAGCTTAAACTATGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCATAGTTTAAGCTGTAAACTCGAGTTTACAGCTTAAACTATGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCATAGTTTAAGCTGTAAACTCGAGTTTACAGCTTAAACTATGGGC-3'

Other clones with same target sequence:

  1. TRCN0000309647