Construct: shRNA TRCN0000097405

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029790.1-561s1c1
DNA Barcode:
GCGGAGGCATTGCTAATGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mettl15 (76894)
Gene Description:
methyltransferase like 15
Transcript:
RefSeq NM_029790.1 (NON-CURRENT)
Match location:
Position 561 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000097405)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGAGGCATTGCTAATGAAA
Hairpin Sequence:
5'-CCGG-GCGGAGGCATTGCTAATGAAA-CTCGAG-TTTCATTAGCAATGCCTCCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGAGGCATTGCTAATGAAACTCGAGTTTCATTAGCAATGCCTCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGAGGCATTGCTAATGAAACTCGAGTTTCATTAGCAATGCCTCCGC-3'

Other clones with same target sequence:

(none)