Construct: shRNA TRCN0000097480

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_201371.1-280s1c1
DNA Barcode:
CGGAACTCCATGTACCATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prmt8 (381813)
Gene Description:
protein arginine N-methyltransferase 8
Transcript:
RefSeq NM_201371.1 (NON-CURRENT)
Match location:
Position 280 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000097480)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAACTCCATGTACCATAAT
Hairpin Sequence:
5'-CCGG-CGGAACTCCATGTACCATAAT-CTCGAG-ATTATGGTACATGGAGTTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAACTCCATGTACCATAATCTCGAGATTATGGTACATGGAGTTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAACTCCATGTACCATAATCTCGAGATTATGGTACATGGAGTTCCG-3'

Other clones with same target sequence:

(none)