Construct: shRNA TRCN0000097485

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025462.1-309s1c1
DNA Barcode:
GCAGCCATGCAAGTTCGCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
1810009K13Rik (66275)
Gene Description:
RIKEN cDNA 1810009K13 gene
Transcript:
RefSeq NM_025462.1 (NON-CURRENT)
Match location:
Position 309 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000097485)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCCATGCAAGTTCGCTAT
Hairpin Sequence:
5'-CCGG-GCAGCCATGCAAGTTCGCTAT-CTCGAG-ATAGCGAACTTGCATGGCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCCATGCAAGTTCGCTATCTCGAGATAGCGAACTTGCATGGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCCATGCAAGTTCGCTATCTCGAGATAGCGAACTTGCATGGCTGC-3'

Other clones with same target sequence:

(none)