Construct: shRNA TRCN0000097525

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172687.1-359s1c1
DNA Barcode:
GCGCCTCTTCATTCTATGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Coq3 (230027)
Gene Description:
coenzyme Q3 methyltransferase
Transcript:
RefSeq NM_172687.1 (CURRENT)
Match location:
Position 359 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000097525)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCCTCTTCATTCTATGAAT
Hairpin Sequence:
5'-CCGG-GCGCCTCTTCATTCTATGAAT-CTCGAG-ATTCATAGAATGAAGAGGCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCCTCTTCATTCTATGAATCTCGAGATTCATAGAATGAAGAGGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCCTCTTCATTCTATGAATCTCGAGATTCATAGAATGAAGAGGCGC-3'

Other clones with same target sequence:

(none)