Construct: shRNA TRCN0000097551

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133788.1-218s1c1
DNA Barcode:
GATAGCCATCAGAGCTTGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Icmt (57295)
Gene Description:
isoprenylcysteine carboxyl methyltransferase
Transcript:
RefSeq NM_133788.1 (NON-CURRENT)
Match location:
Position 218 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000097551)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATAGCCATCAGAGCTTGTTT
Hairpin Sequence:
5'-CCGG-GATAGCCATCAGAGCTTGTTT-CTCGAG-AAACAAGCTCTGATGGCTATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATAGCCATCAGAGCTTGTTTCTCGAGAAACAAGCTCTGATGGCTATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATAGCCATCAGAGCTTGTTTCTCGAGAAACAAGCTCTGATGGCTATC-3'

Other clones with same target sequence:

  1. TRCN0000287907