Construct: shRNA TRCN0000097562

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027334.2-84s1c1
DNA Barcode:
CCCATGTTTCTGCTGAACCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mettl7a1 (70152)
Gene Description:
methyltransferase like 7A1
Transcript:
RefSeq NM_027334.2 (NON-CURRENT)
Match location:
Position 84 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000097562)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATGTTTCTGCTGAACCTT
Hairpin Sequence:
5'-CCGG-CCCATGTTTCTGCTGAACCTT-CTCGAG-AAGGTTCAGCAGAAACATGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATGTTTCTGCTGAACCTTCTCGAGAAGGTTCAGCAGAAACATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATGTTTCTGCTGAACCTTCTCGAGAAGGTTCAGCAGAAACATGGG-3'

Other clones with same target sequence:

  1. TRCN0000335743