Construct: shRNA TRCN0000097591

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026195.1-348s1c1
DNA Barcode:
GCCAGGCTTGATTTCAACCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atic (108147)
Gene Description:
5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase
Transcript:
RefSeq NM_026195.1 (NON-CURRENT)
Match location:
Position 348 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000097591)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGGCTTGATTTCAACCTT
Hairpin Sequence:
5'-CCGG-GCCAGGCTTGATTTCAACCTT-CTCGAG-AAGGTTGAAATCAAGCCTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGGCTTGATTTCAACCTTCTCGAGAAGGTTGAAATCAAGCCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGGCTTGATTTCAACCTTCTCGAGAAGGTTGAAATCAAGCCTGGC-3'

Other clones with same target sequence:

(none)