Construct: shRNA TRCN0000097601

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010321.1-272s1c1
DNA Barcode:
CGCCAGCGACAAGATGCTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gnmt (14711)
Gene Description:
glycine N-methyltransferase
Transcript:
RefSeq NM_010321.1 (CURRENT)
Match location:
Position 272 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000097601)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCAGCGACAAGATGCTGAA
Hairpin Sequence:
5'-CCGG-CGCCAGCGACAAGATGCTGAA-CTCGAG-TTCAGCATCTTGTCGCTGGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCAGCGACAAGATGCTGAACTCGAGTTCAGCATCTTGTCGCTGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCAGCGACAAGATGCTGAACTCGAGTTCAGCATCTTGTCGCTGGCG-3'

Other clones with same target sequence:

(none)