Construct: shRNA TRCN0000097680

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011661.1-2420s1c1
DNA Barcode:
GCGATGGGAAACTATCTGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tyr (22173)
Gene Description:
tyrosinase
Transcript:
RefSeq NM_011661.1 (NON-CURRENT)
Match location:
Position 2420 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000097680)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGATGGGAAACTATCTGAAT
Hairpin Sequence:
5'-CCGG-GCGATGGGAAACTATCTGAAT-CTCGAG-ATTCAGATAGTTTCCCATCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGATGGGAAACTATCTGAATCTCGAGATTCAGATAGTTTCCCATCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGATGGGAAACTATCTGAATCTCGAGATTCAGATAGTTTCCCATCGC-3'

Other clones with same target sequence:

(none)