Construct: shRNA TRCN0000097691

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022565.1-1386s1c1
DNA Barcode:
CCAGTCCATATTCAGTTGTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ndst4 (64580)
Gene Description:
N-deacetylase/N-sulfotransferase (heparin glucosaminyl) 4
Transcript:
RefSeq NM_022565.1 (NON-CURRENT)
Match location:
Position 1386 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000097691)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTCCATATTCAGTTGTAT
Hairpin Sequence:
5'-CCGG-CCAGTCCATATTCAGTTGTAT-CTCGAG-ATACAACTGAATATGGACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTCCATATTCAGTTGTATCTCGAGATACAACTGAATATGGACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTCCATATTCAGTTGTATCTCGAGATACAACTGAATATGGACTGG-3'

Other clones with same target sequence:

(none)