Construct: shRNA TRCN0000097741

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181422.2-1398s1c1
DNA Barcode:
CCAGACACGTATGCAGACTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pkd2l1 (329064)
Gene Description:
polycystic kidney disease 2-like 1
Transcript:
RefSeq NM_181422.2 (NON-CURRENT)
Match location:
Position 1398 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000097741)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGACACGTATGCAGACTTT
Hairpin Sequence:
5'-CCGG-CCAGACACGTATGCAGACTTT-CTCGAG-AAAGTCTGCATACGTGTCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGACACGTATGCAGACTTTCTCGAGAAAGTCTGCATACGTGTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGACACGTATGCAGACTTTCTCGAGAAAGTCTGCATACGTGTCTGG-3'

Other clones with same target sequence:

(none)