Construct: shRNA TRCN0000097782

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019456.1-1082s1c1
DNA Barcode:
GCTACTTTCTTCTGAGAGCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Apbb1ip (54519)
Gene Description:
amyloid beta (A4) precursor protein-binding, family B, member 1 interacting protein
Transcript:
RefSeq NM_019456.1 (NON-CURRENT)
Match location:
Position 1082 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000097782)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTACTTTCTTCTGAGAGCTT
Hairpin Sequence:
5'-CCGG-GCTACTTTCTTCTGAGAGCTT-CTCGAG-AAGCTCTCAGAAGAAAGTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTACTTTCTTCTGAGAGCTTCTCGAGAAGCTCTCAGAAGAAAGTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTACTTTCTTCTGAGAGCTTCTCGAGAAGCTCTCAGAAGAAAGTAGC-3'

Other clones with same target sequence:

(none)