Construct: shRNA TRCN0000097785

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016927.1-1949s1c1
DNA Barcode:
CCGAAGAATTTCATTCAGTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pkd2l2 (53871)
Gene Description:
polycystic kidney disease 2-like 2
Transcript:
RefSeq NM_016927.1 (NON-CURRENT)
Match location:
Position 1949 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000097785)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAAGAATTTCATTCAGTAT
Hairpin Sequence:
5'-CCGG-CCGAAGAATTTCATTCAGTAT-CTCGAG-ATACTGAATGAAATTCTTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAAGAATTTCATTCAGTATCTCGAGATACTGAATGAAATTCTTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAAGAATTTCATTCAGTATCTCGAGATACTGAATGAAATTCTTCGG-3'

Other clones with same target sequence:

(none)