Construct: shRNA TRCN0000097828

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031186.2-1079s1c1
DNA Barcode:
GCGCACACAAATCACAAATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ndst3 (83398)
Gene Description:
N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3
Transcript:
RefSeq NM_031186.2 (NON-CURRENT)
Match location:
Position 1079 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000097828)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCACACAAATCACAAATTT
Hairpin Sequence:
5'-CCGG-GCGCACACAAATCACAAATTT-CTCGAG-AAATTTGTGATTTGTGTGCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCACACAAATCACAAATTTCTCGAGAAATTTGTGATTTGTGTGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCACACAAATCACAAATTTCTCGAGAAATTTGTGATTTGTGTGCGC-3'

Other clones with same target sequence:

(none)